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Save time with a 90-minute ELISA protocol. Try Human Factor XII ELISA Kit (ab192144) from our SimpleStep ELISA® range.
Abcam’s Factor XII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XII concentrations in plasma, serum, milk, urine and cell culture supernatants.
A Factor XII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XII captured in plate.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated Human Factor XII Antibody||1 x 120µl|
|Chromogen Substrate||1 x 8ml|
|Factor XII Microplate (12 x 8 well strips)||1 unit|
|Factor XII Standard||2 vials|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
Standard curve with background signal subtracted (duplicates; +/- SD).
Factor XII measured in undiluted culture supernatants, MCF-7 levels below level of detection (duplicates +/- SD).
Factor XII measured in biological fluids (duplicates +/- SD). Human plasma and serum were tested in the dilution range of 1/100-1/4000; milk, saliva, CSF and urine were diluted 1/1-1/300.
Representative Standard Curve using ab108835.
ab108835 has not yet been referenced specifically in any publications.