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Abcam’s Factor XII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XII concentrations in plasma, serum, milk, urine and cell culture supernatants.
A Factor XII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XII captured in plate.
Save time with a 90-minute ELISA protocol. Try Human Factor XII ELISA Kit (ab192144) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated Human Factor XII Antibody||1 x 120µl|
|Chromogen Substrate||1 x 8ml|
|Factor XII Microplate (12 x 8 well strips)||1 unit|
|Factor XII Standard||2 vials|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108835 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent dilution.|
Standard curve with background signal subtracted (duplicates; +/- SD).
Factor XII measured in undiluted culture supernatants, MCF-7 levels below level of detection (duplicates +/- SD).
Factor XII measured in biological fluids (duplicates +/- SD). Human plasma and serum were tested in the dilution range of 1/100-1/4000; milk, saliva, CSF and urine were diluted 1/1-1/300.
Representative Standard Curve using ab108835.
ab108835 has not yet been referenced specifically in any publications.