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Abcam’s Fas Ligand (Fas-L) Human in vitro ELISA Set is designed for the quantitative measurement of Fas-L in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The Fas L ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure Fas L in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
SPECIFICITY: Recognizes both natural and recombinant soluble human Fas-Ligand RANGE: 62.5pg/ml - 2000pg/ml SENSITIVITY: <12pg/ml
|Components||5 x 96 tests||10 x 96 tests|
|Detection biotinylated anti-Fas Ligand antibody||1 x 0.55ml||2 x 0.55ml|
|Fas Ligand Capture Antibody||1 x 0.5ml||2 x 0.5ml|
|Fas Ligand standard||5 vials||10 vials|
|Ready-to-use TMB||2 vials||4 vials|
|Streptavidin-HRP||1 vial||2 vials|
Our Abpromise guarantee covers the use of ab83703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab83703 has not yet been referenced specifically in any publications.