Overview

  • Product name
    Human Fas Ligand ELISA Set (without plates)
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 44 pg/ml
  • Range
    62.5 pg/ml - 2000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Fas Ligand (Fas-L) Human in vitro ELISA Set is designed for the quantitative measurement of Fas-L in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The Fas L ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA Set is intended to measure Fas L in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    SPECIFICITY: Recognizes both natural and recombinant soluble human Fas-Ligand RANGE: 62.5pg/ml - 2000pg/ml SENSITIVITY: <12pg/ml

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Reagents

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 5 x 96 tests 10 x 96 tests
    Detection biotinylated anti-Fas Ligand antibody 1 x 0.55ml 2 x 0.55ml
    Fas Ligand Capture Antibody 1 x 0.5ml 2 x 0.5ml
    FAS Ligand Standard (Lyophilized) Yellow 5 vials 10 vials
    Ready-to-use TMB 2 vials 4 vials
    Streptavidin-HRP 1 vial 2 vials
  • Research areas
  • Relevance
    Fas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumors. Tumor cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system. The full length of FasL contains 281 amino acids, consisting of the transmembrane domain, extracellular domain, and the cytoplasmic region. A metalloproteinase cleaves the membrane-bound FasL to produce soluble Fas (sFasL), which exists as a homotrimer. The sFasL shows lower cytotoxicity than membrane bound FasL.
  • Cellular localization
    Cell membrane: single pass type II membrane protein. Secreted protein: may be released into the extracellular fluid, probably by cleavage form the cell surface.
  • Alternative names
    • Apoptosis (APO 1) antigen ligand 1
    • APT1LG1
    • APTL
    • CD178
    • CD178 antigen
    • CD95 ligand
    • CD95L protein
    • FAS antigen ligand
    • Fas L
    • Fasl Fas ligand (TNF superfamily member 6)
    • FASLG
    • Generalized lymphoproliferative disease
    • Gld
    • TNFSF6
    • Tumor necrosis factor (ligand) superfamily member 6
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab83703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Fas Ligand standard curve ranging from 62.5 to 2000 pg/mL

References

ab83703 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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