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ab118182 uses quantitative immunocytochemistry to measure protein levels or post-translational modifications in cultured cells. Cells are fixed in a microplate and targets of interest are detected with highly specific, well-characterized monoclonal antibodies, and levels are quantified with IRDye®-labeled Secondary Antibodies. IR imaging and quantitation is performed using a LI-COR® Odyssey® or Aerius® system.
Plates are available in our ICE (In-Cell ELISA) Support Pack (ab111542) which can be bought seperately.
Upon receipt spin down the contents of the IRDye®-labeled Secondary Antibody tube and protect from light. Store all components upright at 4C. This kit is stable for at least 6 months from receipt.
|Components||1 x 96 tests|
|1000X IRDye 800-labeled Secondary antibody||1 x 0.036ml|
|100X ACADM Primary Antibody||1 x 0.12ml|
|100X ACADVL Primary Antibody||1 x 0.12ml|
|100X HADHA Primary Antibody||1 x 0.12ml|
|10X Blocking Buffer||1 x 20ml|
|10X Phosphate Buffered Saline||1 x 100ml|
|33x Triton X-100||1 x 5ml|
|400X Tween-20||1 x 2ml|
|Janus Green Stain||1 x 17ml|
Our Abpromise guarantee covers the use of ab118182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|In-Cell ELISA||Use at an assay dependent concentration.|
ab118182 has not yet been referenced specifically in any publications.