Overview

  • Product name
    Human Fc-gamma RIIB/C ELISA Kit
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 85 pg/ml
  • Range
    78.13 pg/ml - 5000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 88.89 79% - 99%
    Serum 100 91% - 108%
    Plasma 112.1 96% - 135%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Fc-gamma RIIB/C (Fc gamma Receptor II B/C) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fc-gamma RIIB/C in serum, plasma, cell culture supernatants.

    This assay employs an antibody specific for Human Fc-gamma RIIB/C coated on a 96- well plate. Standards and samples are pipetted into the wells and Fc-gamma RIIB/C present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Fc-gamma RIIB/C antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fc-gamma RIIB/C bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab113333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab113333

  • Representative Standard Curve using ab113333

Protocols

References

ab113333 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab113333.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up