|Sample type||Average %||Range|
|Cell culture supernatant||88.89||79% - 99%|
|Serum||100||91% - 108%|
|Plasma||112.1||96% - 135%|
Abcam’s Fc-gamma RIIB/C (Fc gamma Receptor II B/C) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fc-gamma RIIB/C in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human Fc-gamma RIIB/C coated on a 96- well plate. Standards and samples are pipetted into the wells and Fc-gamma RIIB/C present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Fc-gamma RIIB/C antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fc-gamma RIIB/C bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|200X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human Fc-gamma RIIB/C||2 vials|
|Fc-gamma RIIB/C Microplate (12 strips x 8 wells)||1 unit|
|Recombinant Human Fc-gamma RIIB/C Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab113333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab113333 has not yet been referenced specifically in any publications.