Abcam’s Ferritin competitive Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Ferritin in serum and plasma.
A 96-well plate has been precoated with anti-Ferritin IgG antibodies. Samples, standards and the Ferritin-HRP conjugate are added to the wells, where Ferritin in the sample and standards binds to the precoated antibody and added Ferritin-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Ferritin in the sample and the intensity is measured at 450 nm.
Get results in 90 minutes with Human Ferritin ELISA Kit (FLT) (ab200018) from our SimpleStep ELISA® range.
|Components||Identifier||1 x 96 tests|
|10X Washing Solution||1 x 50ml|
|Anti-Ferritin IgG Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Anti-Ferritin-HRP conjugate||1 x 12ml|
|Cover foil||1 unit|
|Ferritin Control||1 x 1ml|
|Ferritin Standard 0 - 0 ng/mL||1 x 3ml|
|Ferritin Standard 1 - 5 ng/mL||1 x 1ml|
|Ferritin Standard 2 - 20 ng/mL||1 x 1ml|
|Ferritin Standard 3 - 100 ng/mL||1 x 1ml|
|Ferritin Standard 4 - 400 ng/mL||1 x 1ml|
|Ferritin Standard 5 - 1,000 ng/mL||1 x 1ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab108698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
ab108698 has not yet been referenced specifically in any publications.