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Abcam’s Ferritin (FTL) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ferritin concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture supernatants.
A Ferritin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ferritin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ferritin captured in plate.
Save time with a 90-minute ELISA protocol. Try Human Ferritin ELISA Kit (FLT) (ab200018) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent M Concentrate||1 x 20ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated Human Ferritin Antibody||1 x 120µl|
|Chromogen Substrate||1 x 8ml|
|Ferritin Microplate (12 x 8 well strips)||1 unit|
|Ferritin Standard||1 vial|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
Human Ferritin measured in biological fluids showing quantity (pg) per mL of tested sample. Serum and plasma samples were diluted 10-20 fold. Milk, urine and PBMC supernatant were diluted 1-4 fold.
Representative Standard Curve using ab108837