Overview

  • Product nameHuman FGF basic ELISA Kit (FGF2)
    See all FGF basic kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Cell Lysate
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 50 pg/ml
  • Range
    102.4 pg/ml - 10000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.48 83% - 103%
    Serum 95.46 84% - 104%
    Plasma 92.64 82% - 103%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Product overview

    Abcam’s FGF basic (FGF2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF basic in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceFGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
  • Alternative names
    • Basic fibroblast growth factor
    • BFGF
    • FGF 2
    • FGF B
    • FGF2
    • FGF2 basic
    • FGFB
    • Fibroblast growth factor 2 (basic)
    • HBGF 2
    • HBGF2
    • HBGH 2
    • Heparin binding growth factor 2
    • Heparin binding growth factor 2 precursor
    • OTTMUSP00000046776
    • Prostatropin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human FGF basic ELISA Kit (FGF2) images

  • Representative Standard Curve using ab99979.

  • Representative Standard Curve using ab99979.

Protocols

References for Human FGF basic ELISA Kit (FGF2) (ab99979)

ab99979 has not yet been referenced specifically in any publications.

Product Wall

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Leider hat das Labornicht einpräzipitiertes FGF getestet in diesem ELISA, weder ein FGF präzipitiert mit Ammonium Sulfat oder Säure. Das Labor denkt auch, dass 10kD cut off (oder 5kD cut off) s...

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Thank you for contacting us with your enquiry, and for your patience while I have been in touch with the lab for more information.

We have not tested any of these kits in-house with cerebrospinal fluid, but the lab scientistsrecommend using a...

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These kits are provided with 12 strips of 8 wells each in a frame that is the same size as a standard 96-well plate (˜5 x 3.33"). If you need any more specific information, please let me know and I will be happy t...

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This kit is suitable for FGF basic detection in serum, plasma, cell culture medium and urine.

The kit is sensitive to detect the FGFbasic as lower than 50pg/ml. If customer is interested in testing the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"