Human FGF basic ELISA Kit (FGF2) (ab99980)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 102.4 pg/ml - 10000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human FGF basic ELISA Kit (FGF2)
See all FGF basic kits -
Detection method
Colorimetric -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
< 50 pg/ml -
Range
102.4 pg/ml - 10000 pg/ml -
Recovery
> 95 %
Sample specific recovery Sample type Average % Range Cell culture extracts 97.65 87% - 108% Tissue Extracts 95.87 86% - 107% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s FGF basic (FGF2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF basic in cell lysates and tissue lysates.
This assay employs an antibody specific for Human FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml Biotinylated anti-Human FGF basic 2 vials FGF basic Microplate (12 x 8 wells) 1 unit Recombinant Human FGF basic Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. -
Alternative names
- Basic fibroblast growth factor
- BFGF
- FGF 2
see all -
Database links
- Entrez Gene: 2247 Human
- Omim: 134920 Human
- SwissProt: P09038 Human
Images
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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FGF2 measured in tissue lysates showing quantity (pg) per 1 mg of extracted protein. Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were diluted 200-2000 fold.
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FGF2 measured in cell culture lysates showing quantity (pg) per 1 mln cells. Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 200-2000 fold.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab99980 has been referenced in 1 publication.
- Jaszberenyi M et al. Potentiation of cytotoxic chemotherapy by growth hormone-releasing hormone agonists. Proc Natl Acad Sci U S A 111:781-6 (2014). PubMed: 24379381