Overview

  • Product name
    Human FGF9 ELISA Kit
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 200 pg/ml
  • Range
    358.4 pg/ml - 35000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100.27 88% - 107%
    Plasma 94.38 83% - 102%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s FGF9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF9 in plasma and cell culture supernatants. (Human FGF9 concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human FGF9 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Human FGF9 in normal serum with ab100520, only in serum spiked with Human FGF9.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human FGF9 2 vials
    FGF9 Microplate (12 strips x 8 wells) 1 unit
    Recombinant Human FGF9 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
  • Tissue specificity
    Glial cells.
  • Involvement in disease
    Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [MIM:612961]. Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness.
  • Sequence similarities
    Belongs to the heparin-binding growth factors family.
  • Post-translational
    modifications
    Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.
    N-glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Elbow knee synostosis
    • FGF 9
    • FGF-9
    • FGF9
    • FGF9_HUMAN
    • Fibroblast growth factor 9
    • GAF
    • Glia Activating Factor
    • Glia-activating factor
    • HBFG 9
    • HBFG9
    • HBGF-9
    • Heparin binding growth factor 9
    • Heparin-binding growth factor 9
    • MGC119914
    • MGC119915
    • SYNS3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100520.

  • Representative Standard Curve using ab100520.

Protocols

References

ab100520 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your reply. The microplate is from Greiner, cat# 762071. This should allow you to find any specific dimension information needed. Please let me know if you have any further questions.

Thank you for contacting us.

These kits are provided with 12 strips of 8 wells each in a frame that is the same size as a standard 96-well plate (˜5 x 3.33"). If you need any more specific information, please let me know and I will be happy t...

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Thank you for contacting Abcam earlier today.

I have spoken to the lab and they have advised that you should not store the detection antibody concentrate for longer than 5 days at 4ºC. While not ideal, if you absolutely need to store it ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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