• Product nameHuman Fibronectin ELISA Kit
    See all Fibronectin kits
  • Detection methodColorimetric
  • Precision
    Sample n Mean SD CV%
    Overall 4.9%
    Sample n Mean SD CV%
    Overall 7%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 4 ng/ml
  • Range
    0.004 µg/ml - 1 µg/ml
  • Recovery

    97.5 %

  • Assay time
    3h 30m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Fibronectin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibronectin levels in urine, saliva, milk, cerebrospinal fluid and cell culture supernatants.

    A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in diseaseGlomerulopathy with fibronectin deposits 2
  • Sequence similaritiesContains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localizationSecreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Alternative names
    • CIG
    • Cold insoluble globulin
    • Cold-insoluble globulin
    • DKFZp686F10164
    • DKFZp686H0342
    • DKFZp686I1370
    • DKFZp686O13149
    • ED B
    • Fibronectin 1
    • FINC
    • FN
    • FN1
    • FNZ
    • GFND
    • GFND2
    • LETS
    • Migration stimulating factor
    • MSF
    • Ugl-Y3
    see all
  • Database links


Our Abpromise guarantee covers the use of ab108847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human Fibronectin ELISA Kit images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Fibronectin measured in biological fluids and cell culture supernatants showing quantity (ug) per mL of tested sample. Human urine diluted 1-3 fold. Human saliva diluted 10-100 fold. Human milk diluted 100-1000 fold. Cell supernatants diluted 10-1000 fold.


References for Human Fibronectin ELISA Kit (ab108847)

This product has been referenced in:
  • Jagadeeshan S  et al. Transcriptional regulation of fibronectin by p21-activated kinase-1 modulates pancreatic tumorigenesis. Oncogene N/A:N/A (2014). Read more (PubMed: 24561527) »

See 1 Publication for this product

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Je me réjouis de recevoir les résultats que vous avez obtenu avec ce kit, afin de pouvoir consulter avec le laboratoire et mieux vous conseiller.

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Thank you for your enquiry.

I am sorry to confirm that ab108847 Fibronectin Human ELISA Kithas not been tested on plasma or serum, and we could not guarantee it for these sample types.

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