|Sample type||Average %||Range|
|Serum||110.6||% - %|
Abcam’s Fibronectin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human fibronectin concentrations in cell culture supernatant, serum, plasma (EDTA, heparin) and urine.
Fibronectin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an fibronectin biotinylated detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using was buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of fibronectin captured on the plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||3 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-human Fibronectin polyclonal antibody||1 x 70µl|
|Fibronectin Standard lyophilized (40 ng/mL upon reconstitution)||2 vials|
|Microplate coated with polyclonal antibody to fibronectin (12 x 8 wells)||1 unit|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 200µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119524 has not yet been referenced specifically in any publications.