Human FMRP (phospho S499) peptide (ab48658)

Overview

Description

  • NatureSynthetic

Specifications

Our Abpromise guarantee covers the use of ab48658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-FMRP (phospho S499) antibody (ab48127)

  • Purity70 - 90% by HPLC.

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • FMR 1
    • Fmr1
    • Fmr1 gene
    • FMR1_HUMAN
    • FMRP
    • Fragile X mental retardation 1
    • Fragile X mental retardation 1 protein
    • Fragile X mental retardation protein
    • Fragile X mental retardation protein 1
    • fragile X mental retardation syndrome-related protein 1
    • fragile X mental retardation, autosomal homolog 1
    • FRAXA
    • fxr1
    • MGC87458
    • POF
    • POF1
    • Protein FMR-1
    • Protein FMR1
    • wu:fb16f11
    • wu:fd18c10
    • zgc:66226
    see all
  • FunctionTranslation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
  • Tissue specificityHighest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells.
  • Involvement in diseaseDefects in FMR1 are the cause of fragile X syndrome (FRAX) [MIM:300624]. Fragile X syndrome is a common genetic disease (has a prevalence of one in every 2000 children) which is characterized by moderate to severe mental retardation, macroorchidism (enlargement of the testicles), large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
    Defects in FMR1 are the cause of fragile X tremor/ataxia syndrome (FXTAS) [MIM:300623]. In FXTAS, the expanded repeats range in size from 55 to 200 repeats and are referred to as 'premutations'. Full repeat expansions with greater than 200 repeats results in fragile X mental retardation syndrome [MIM:300624]. Carriers of the premutation typically do not show the full fragile X syndrome phenotype, but comprise a subgroup that may have some physical features of fragile X syndrome or mild cognitive and emotional problems.
    Defects in FMR1 are the cause of premature ovarian failure syndrome type 1 (POF1) [MIM:311360]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similaritiesBelongs to the FMR1 family.
    Contains 2 KH domains.
  • Post-translational
    modifications
    Phosphorylated on several serine residues.
  • Cellular localizationCytoplasm. Nucleus > nucleolus.
  • Information by UniProt

References for Human FMRP (phospho S499) peptide (ab48658)

ab48658 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam. The peptide ab48658 is phosphorylated at reside 499 and the other phospho residues at 501 and 503 are non-phosphorylated in this peptide. The peptide ab48659 is the same sequence as the ab48658 peptide bu...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"