Human Follistatin ELISA Kit (ab113319)

Overview

  • Product nameHuman Follistatin ELISA Kit
    See all Follistatin kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.5 ng/ml
  • Range
    0.512 ng/ml - 125 ng/ml
  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 107.3 102% - 113%
    Serum 86.98 81% - 92%
    Plasma 75.78 67% - 83%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Follistatin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Follistatin in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human Follistatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Follistatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Follistatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Follistatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Save time with a 90-minute ELISA protocol. Try Human Follistatin ELISA Kit (ab192147) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab113319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Follistatin ELISA Kit images

  • Representative standard curve using ab113319

Protocols

References for Human Follistatin ELISA Kit (ab113319)

ab113319 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
We trialed the Follistatin Human ELISA kit for Mouse Serum Samples to determine its effectiveness in detecting mouse follistatin. The kit was performed according to Abcam's protocol and guidelines. We found that the Follistatin Human ELISA kit is not effective in detecting mouse follistatin. While the human standards demonstrated a good standard curve, the mouse standards, which were created using recombinant mouse follistatin (R&D Systems), did not show much difference between the maximum and minimum concentrations. Additionally, there was a precipitate that formed in the mouse standards that was not identifiable and interfered with the absorbance reading.
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Verified customer

Submitted Nov 11 2014

Thank you for your enquiry. I am sorry it has taken some time to confirm this information for you. I can confirm that heparin-plasma is compatible with ab113319 Follistatin Human ELISA Kit. The kit is compatible with heparin, EDTA, and citrate anti...

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Thank you for contacting us.  The Follistatin ELISA kit ab113319 is compatible with heparin, EDTA, and citrate anti-coagulants.  I hope this helps, please let me know if you need any additional information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"