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|Sample type||Average %||Range|
|Serum||110||99% - 125%|
|Cell culture media||86||85% - 86%|
|EDTA Plasma||92||89% - 95%|
Follistatin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Follistatin protein in human serum, plasma and cell culture supernatant samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Follistatin is a single-chain polypeptide consisting of about 344 amino acids. The follistatin protein is encoded by a relatively small gene size of 6 kb and 6 exons with alternative splice variants and post translational modifications to give a variety of molecular weights between 31 – 39 kDa. Follistatin forms a high affinity complex with activin sequestering it from its receptor thus having an inhibitory effect. Follistatin acts to promote muscle growth in mammals by acting as an antagonist to the protein myostatin. Myostatin acts as an inhibitor to muscle growth.
|Components||1 x 96 tests|
|10X Follistatin Capture Antibody||1 x 600µl|
|10X Follistatin Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|Antibody Diluent 5BI||1 x 6ml|
|Follistatin Human Lyophilized Recombinant Protein||2 vials|
|Plate Seals||1 unit|
|Sample Diluent 25BP||1 x 20ml|
|Sample Diluent NS (ab193972)||1 x 50ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab192147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab192147 has not yet been referenced specifically in any publications.