Overview

Description

  • Nature
    Synthetic

Specifications

Our Abpromise guarantee covers the use of ab16809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-FOXP3 antibody [mAbcam 450] (ab450), Anti-FOXP3 antibody (ab10901), Anti-FOXP3 antibody [mAbcam 22510] (ab22510), Anti-FOXP3 antibody [mAbcam 22510] (HRP) (ab22831)

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • AIID
    • DIETER
    • Forkhead box P3
    • Forkhead box protein P3
    • FOXP3
    • FOXP3_HUMAN
    • FOXP3delta7
    • Immune dysregulation polyendocrinopathy enteropathy X linked
    • Immunodeficiency polyendocrinopathy enteropathy X linked
    • IPEX
    • JM2
    • MGC141961
    • MGC141963
    • OTTHUMP00000025832
    • OTTHUMP00000025833
    • OTTHUMP00000226737
    • PIDX
    • Scurfin
    • XPID
    see all
  • Function
    Probable transcription factor. Plays a critical role in the control of immune response.
  • Involvement in disease
    Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • Sequence similarities
    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • All lanes : Anti-FOXP3 antibody (ab10901) at 0.4 µg/ml

    Lane 1 : 20µg HEK 293 lysate
    Lane 2 : 20µg HEK 293 lysate over expressing human FOXP3
    Lane 3 : 20µg HEK 293 lysate over expressing mouse FOXP3
    Lane 4 : 20µg HEK 293 lysate with Human FOXP3 peptide (ab16809) at 1 µg/ml
    Lane 5 : 20µg HEK 293 lysate over expressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml
    Lane 6 : 20µg HEK 293 lysate over expressing mouse FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml

    Secondary
    Lanes 4 - 6 : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 0.2 µg/ml


    Performed under reducing conditions.

    Predicted band size : 47 kDa


    Exposure time : 1 minute

    A no primary control lane showed no bands.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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