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Abcam’s Free Estriol competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Free Estriol in serum and plasma.
A 96-well plate has been precoated with anti-Estriol IgG. Samples and the Estriol-HRP conjugate are added to the wells, where Estriol in the sample competes with the added Estriol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estriol in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|anti-Estriol IgG Coated Microplate (12 x 8 wells)||1 x 98 tests|
|10X Wash Solution||1 x 50ml|
|Estriol HRP Conjugate||1 x 15ml|
|Estriol Control||1 x 1ml|
|Estriol Standard 0.15 ng/mL||1 x 1ml|
|Estriol Standard 0.75 ng/mL||1 x 1ml|
|Estriol Standard 15.0 ng/mL||1 x 1ml|
|Estriol Standard 0 ng/mL||1 x 1ml|
|Estriol Standard 2.0 ng/mL||1 x 1ml|
|Estriol Standard 40.0 ng/ml||1 x 1ml|
|Estriol Standard 5.0 ng/ml||1 x 1ml|
|Stop Solution||1 x 15ml|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab108671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent dilution.|
ab108671 has not yet been referenced specifically in any publications.