Human Free Prostate Specific Antigen ELISA Kit (ab188389)

Overview

  • Product name
    Human Free Prostate Specific Antigen ELISA Kit
    See all Prostate Specific Antigen kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Urine 8 2.5%
    Inter-assay
    Sample n Mean SD CV%
    Urine 3 4.7%
  • Sample type
    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    4.9 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 105 105% - 106%
    Serum 81 81% - 82%
    Cell culture media 93 91% - 94%
    Heparin Plasma 83 77% - 86%
    EDTA Plasma 87 84% - 90%
    Citrate Plasma 83 82% - 86%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Hamster, Cow, Dog, Pig
  • Product overview

    Free Prostate Specific Antigen (free PSA) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Free Prostate Specific Antigen protein in human serum, plasma, urine and cell culture supernatant samples.


    The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Prostate specific antigen (PSA) or also commonly termed tissue Kallikrein-3 (KLK3) is a 34 kD serine protease from the Kallikrein family. PSA has very high expression in the prostate gland but expression also has been reported in the breast and saliva. PSA functions by hydrolyzing semenogelin-1 leading to the liquefaction of the seminal coagulum. This is essential for the proper release of sperm during ejaculation. The majority (>70%) of PSA is known to form a complex with alpha-1-antichymotrypsin (PSA-ACT) in the blood. The remaining PSA exists as an unbound inactive enzyme known as Free PSA. PSA is best characterized as a clinical biomarker for Prostate Cancer. During prostate cancer progression, excess amounts of PSA are leaked into the blood dramatically elevating both Total and Free PSA levels.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab188389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 3) are graphed.

  • Mean Human male Free PSA is 50.2 pg/mL or 11% Free PSA and values fall within expected normal reference ranges (WHO). Observed Total PSA levels were also determined using the Total Prostate Specific Antigen SimpleStep ELISA ab188388. Mean Human male Total PSA is 424 pg/mL and values fall within expected normal reference ranges (WHO).

  • Mean Human female Free PSA is 12.7 pg/mL (40% detectable) and values fall within expected normal reference ranges (WHO). Observed Total PSA levels were also determined using the Total Prostate Specific Antigen SimpleStep ELISA ab188388. Mean Human female Total PSA is 12.5 pg/mL and values fall within expected normal reference ranges (WHO).

  • Mean Human Free PSA values fall within expected normal reference ranges (WHO). Observed Total PSA levels were also determined using the Total Prostate Specific Antigen SimpleStep ELISA ab188388. Mean Human Total PSA values fall within expected normal reference ranges (WHO). Please note: overall Total and Free PSA levels are lower due to mixed gender samples.

  • Mean Human Free PSA is 61.9 ng/mL and values fall within expected normal reference ranges (WHO). Observed Total PSA levels were also determined using the Total Prostate Specific Antigen SimpleStep ELISA ab188388. Mean Human Total PSA values are 71.7 ng/mL and fall within expected normal reference ranges (WHO).

Protocols

References

ab188389 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Free-PSA ELISA kit for cell lysates

Average Good 4/5 (Ease of Use)
Abreviews
This Abreview shows the results of the quantification of free-PSA from LNCap and C4-2b cell lysates using the free PSA ELISA kit (ab188389).
The design of experiment included the quantification of PSA in cell supernatant, cell lysate and the combination of the two (cell supernatant + cell lysate). Theroretically, if the kit can be used to measure free-PSA concentrations in cell lysates, then the obtained value for the sample "supernatant + lysate" should be the same as the sum of the individual values obtained for the samples "supernatant" and "cell lysate".
For LNCap cells, the obtained value for free-PSA concentration in "cell lysate" was out of the linear range of the assay and therefore any conclusions taken for this cells should be carefully taken. In the case of C4-2b cells, values were within the linear range of the assay and while the obtained value for the free-PSA in the sample "supernatant + cell lysate" was 1152857 pg/ml, the theoretical value that resulted from the sum of the values obtained for samples "supernatant" and "cell lysate" was 1770714 pg/ml. The ratio "real"/"theoretical" is 0.7.
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Ms. Sara Pereira

Verified customer

Submitted Sep 09 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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