Overview

  • Product nameHuman Furin ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    13.72 pg/ml - 10000 pg/ml
  • Recovery

    84 %

    Sample specific recovery
    Sample type Average % Range
    Serum 89.57 80% - 98%
    Plasma 77.74 69% - 85%
    Cell culture media 84.69 75% - 93%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Furin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Furin in serum, plasma (collect plasma using heparin and EDTA as an anticoagulant. Citrate is not recommended) and cell culture supernatants.

    This assay employs an antibody specific for Human Furin coated on a 96-well plate. Standards and samples are pipetted into the wells and Furin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Furin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Furin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab113322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Furin ELISA Kit images

  • Representative standard curve using ab113322

  • Representative standard curve using ab113322

Protocols

References for Human Furin ELISA Kit (ab113322)

ab113322 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

This kit has not been designed nor validated with tissue lysate samples. However it is very accommodating to many different samples types, so lysates will most likely work. If you decide to test this kit with tiss...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"