Recombinant human Galectin 3 protein (Active) (ab89487)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human Galectin 3 protein (Active)
See all Galectin 3 proteins and peptides -
Biological activity
The ED50 for this effect is ≤ 15 ug/ml.
Measured by its ability to agglutinate human red blood cells.
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Purity
> 95 % SDS-PAGE.
Purified by using conventional chromatography techniques -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPGQAP PGAYPGQAPP GAYPGAPGAY PGAPAPGVYP GPPSGPGAYP SSGQPSATGA YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI QVLVEPDHFK VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI DLTSASYTMI -
Predicted molecular weight
28 kDa including tags -
Amino acids
1 to 250 -
Tags
His tag N-Terminus -
Additional sequence information
(NCBI NP_002297) MW confirmed by MALDI-TOF.
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Associated products
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Related Products
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Galectin 3 antibody [A3A12] (ab2785)
- Anti-Galectin 3 antibody (ab31707)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Galectin 3 antibody (ab53082)
- Anti-Galectin 3 antibody [EP2775Y] (ab76245)
- Anti-Galectin 3 antibody [EPR2774] (ab76466)
- Biotin Anti-Galectin 3 antibody (ab84238)
- Anti-6X His tag® antibody (ab9108)
Specifications
Our Abpromise guarantee covers the use of ab89487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 35 kDa lectin
- Carbohydrate binding protein 35
- Carbohydrate-binding protein 35
see all -
Function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. -
Tissue specificity
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab89487 has been referenced in 3 publications.
- Mathew MP et al. Nutrient-responsive O-GlcNAcylation dynamically modulates the secretion of glycan-binding protein galectin 3. J Biol Chem 298:101743 (2022). PubMed: 35183508
- Stasenko M et al. Targeting galectin-3 with a high-affinity antibody for inhibition of high-grade serous ovarian cancer and other MUC16/CA-125-expressing malignancies. Sci Rep 11:3718 (2021). PubMed: 33580170
- Jiang K et al. Galectin-3 regulates desmoglein-2 and intestinal epithelial intercellular adhesion. J Biol Chem 289:10510-7 (2014). PubMed: 24567334