Human Galectin-3BP ELISA Kit (ab213784)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Galectin-3BP ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 312pg/ml 18.72 = 6% 2 16 1090pg/ml 63.22 = 5.8% 3 16 4158pg/ml 282.74 = 6.8% Inter-assay Sample n Mean SD CV% 1 24 326pg/ml 20.86 = 6.4% 2 24 1154pg/ml 84.24 = 7.3% 3 24 3768pg/ml 271.29 = 7.2% -
Sample type
Cell culture supernatant, Serum, Hep Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human Galectin-3BP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213784) is designed for the quantitative measurement of Human Galectin-3BP in cell culture supernatants, serum and plasma (heparin).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-3BP has been pre-coated onto 96-well plates. Standards (NSO; V19-D585) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Galectin-3BP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Galectin-3BP amount of sample captured in plate.
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Notes
Galectin-3-binding protein is a protein that in humans is encoded by the LGALS3BP gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell–cell and cell–matrix interactions. Using fluorescence in situ hybridization, the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage associated lectin known as Mac-2 and also binds to galectin 1.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Adhesive Plate Seal 4 units 4 units Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Human Galectin-3BP coated Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti- Human Galectin-3BP antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Human Galectin-3BP standard 2 vials 2 vials Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
Research areas
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Function
Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells. -
Tissue specificity
Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts. -
Sequence similarities
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 1 SRCR domain. -
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- 90K
- 90K MAC 2 BP
- Basement membrane autoantigen p105
see all -
Database links
- Entrez Gene: 3959 Human
- Omim: 600626 Human
- SwissProt: Q08380 Human
- Unigene: 514535 Human
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab213784 has been referenced in 1 publication.
- Chang TT et al. Plasma proteome plus site-specific N-glycoprofiling for hepatobiliary carcinomas. J Pathol Clin Res 5:199-212 (2019). PubMed: 31136099