Human Galectin-4 ELISA Kit (ab213785)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 31.2 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Galectin-4 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 209pg/ml 11.7 = 7.6% 2 16 1291pg/ml 98.11 = 7.6% 3 16 4066pg/ml 223.63 = 5.5% Inter-assay Sample n Mean SD CV% 1 24 215pg/ml 12.04 = 5.6% 2 24 1324pg/ml 108.56 = 8.2% 3 24 4394pg/ml 320.76 = 7.3% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
31.2 pg/ml - 2000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human Galectin-4 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213785) is designed for the quantitative measurement of Human Galectin-4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-4 has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: A2-I323) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Galectin-4 amount of sample captured in plate.
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Notes
Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly under expressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human Galectin-4 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human Galectin-4 antibody 1 x 100µl Lyophilized recombinant Human Galectin-4 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions. -
Sequence similarities
Contains 2 galectin domains. -
Domain
Contains two homologous but distinct carbohydrate-binding domains. - Information by UniProt
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Alternative names
- Antigen NY CO 27
- Antigen NY-CO-27
- Antigen NYCO27
see all -
Database links
- Entrez Gene: 3960 Human
- Omim: 602518 Human
- SwissProt: P56470 Human
- Unigene: 5302 Human
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab213785 has been referenced in 1 publication.
- Abdel-Tawab MS et al. Evaluation of Serum and Gene Expression of Galectin-4, Interleukin-27, and Complement-7 in Hepatitis C Virus-Infected Egyptian Patients. Biomed Res Int 2020:8879758 (2020). PubMed: 33381596