Human Galectin 7 ELISA Kit (GAL7) (ab100523)

Overview

  • Product nameHuman Galectin 7 ELISA Kit (GAL7)
    See all Galectin 7 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    13.72 pg/ml - 10000 pg/ml
  • Recovery

    > 90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.55 87% - 105%
    Serum 107.1 95% - 115%
    Plasma 91.82 70% - 125%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Galectin 7 (GAL7) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Galectin 7 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Galectin 7 coated on a 96-well plate. Standards and samples are pipetted into the wells and Galectin 7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Galectin 7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceGalectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Galectin 7 is thought to be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Galectin 7 has been implicated in apoptosis and is believed to upregulate MMP9 and as such be involved in the progression of tumorigenesis.
  • Alternative names
    • GAL7
    • HKL14
    • Human keratinocyte lectin 14
    • Lectin galactoside binding soluble 7
    • LGALS7
    • LGALS7B
    • MGC151215
    • MGC151217
    • OTTMUSP00000025970
    • OTTMUSP00000038956
    • p53 induced gene 1 protein
    • PI7
    • PIG1
    • TP53I1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Galectin 7 ELISA Kit (GAL7) images

  • Representative Standard Curve using ab100523.

  • Representative Standard Curve using ab100523.

Protocols

References for Human Galectin 7 ELISA Kit (GAL7) (ab100523)

ab100523 has not yet been referenced specifically in any publications.

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I have had the opportunity to speak with the lab, unfortunately, the reagents used in the diluents and buffers are proprietary and therefore unavailable. They recommend you use the wash buffer provided (Item ...

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Thank you for contacting us. Although the immunogen sequence for this antibody is commercially available, I performed a BLAST between the whole human protein sequence and the whole mouse protein sequence. The percentage of homology obtained is ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"