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|Low conc||16||278pg/ml||= 8.8%|
|Medium conc||16||471pg/ml||= 3.7%|
|High conc||16||920pg/ml||= 5.7%|
|Low conc||8||278pg/ml||= 4.4%|
|Medium conc||8||328pg/ml||= 6.5%|
|High conc||8||1065pg/ml||= 3.4%|
|Sample type||Average %||Range|
|Serum||= 101.9||% - %|
|Tissue Culture Media||= 100.6||% - %|
|Citrate Plasma||= 107.9||% - %|
Abcam’s Gastrin 1 (GAST) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Gastrin 1 (GAST) in tissue culture media, Human serum and Plasma (citrate).
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Gastrin 1 antigen and a polyclonal rabbit antibody specific to Gastrin 1. After incubation the excess reagents are washed away. pNpp substrate is added and catalyzed by AP to produce a yellow color. The optical density of the yellow coloration at 405 nm is inversely proportional to the amount of Gastrin 1 captured in the plate.
Gastrins are a family of sequence-related carboxyamidated peptides produced by endocrine G Cells of the antrum mucosa in response to a number of stimuli associated with digestion. Antral distension, partially digested proteins, amino acids, and vagal stimulation resulting from smelling, tasting, chewing or swallowing food all contribute to gastrin release from G Cell storage. In addition, caffeine, alcohol, hypoglycemia, antacids and elevated calcium levels will also stimulate gastrin release. Increased serum gastrin levels are associated with duodenal ulcers, Helicobacter pylori infections, colorectal carcinomas, and other tumors and cancerous lesions. Gastrin is the most potent stimulator of gastric acid secretion.
Gastrin-I (G17): H2N-Glu-Gly-Pro-Trp-Leu-Glu-Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2
Gastrin is synthesized as a 101 residue pre-pro-peptide on the rough endoplasmic reticulum, then post-translationally modified by cleavage and alpha-amidation to result in the active forms G34, G17 and G13/14; Big, Little and Mini-Gastrins respectively. Other forms also exist, but are not considered biologically significant. There are two types of G17 and G34, type II is sulfated at the tyrosine12 residue, while type I is not. Both G34 and G17 circulate and contribute to the stimulation of gastric acid secretion, but have different clearance rates. In man, G17 has a circulating half-life of about 9 minutes while G34 has a half-life of about 35 minutes.
G34 is the major circulating gastrin in fasting serum, but with G17, increases two to three-fold after feeding until both are present in approximately equal amounts. Fasting gastrin concentration is usually less than 30 pmol/l (equivalent to 62.9 pg/ml) while patients with Zollinger-Ellison Syndrome and gastrinomas have levels exceeding 1,000 pg/ml.
|Compound||% Cross Reactivity|
|Human Gastrin I (G17-I)||100|
|Rat Gastrin I||70.7|
|Gastrin II (G17-II, sulfated)||9.3|
|Cholecystokinin 26-33 (CCK-8)||2.67|
|Gastrin Tetrapeptide (CCK-4)||1.6|
|Big Gastrin (G34-I)||0.8|
|Gastrin Releasing Peptide (GRP)||<0.001|
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 30ml|
|Assay Buffer||1 x 30ml|
|Gastrin I Antibody||1 x 6ml|
|Gastrin I Alkaline Phosphatase Conjugate||1 x 6ml|
|Goat anti-rabbit IgG Microplate (12 x 8 wells)||1 unit|
|Human Gastrin I Standard||1 x 0.5ml|
|pNpp Substrate||1 x 20ml|
|Stop Solution||1 x 5ml|
Our Abpromise guarantee covers the use of ab133033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
ab133033 has not yet been referenced specifically in any publications.