Human Gastrin I ELISA Kit (GAST) (ab133033)

Overview

  • Product name
    Human Gastrin I ELISA Kit (GAST)
    See all Gastrin I kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Low conc 16 278pg/ml = 8.8%
    Medium conc 16 471pg/ml = 3.7%
    High conc 16 920pg/ml = 5.7%
    Inter-assay
    Sample n Mean SD CV%
    Low conc 8 278pg/ml = 4.4%
    Medium conc 8 328pg/ml = 6.5%
    High conc 8 1065pg/ml = 3.4%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    7.27 pg/ml
  • Range
    39.1 pg/ml - 10000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum = 101.9 % - %
    Tissue Culture Media = 100.6 % - %
    Citrate Plasma = 107.9 % - %

  • Assay time
    5h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Gastrin 1 (GAST) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Gastrin 1 (GAST) in tissue culture media, Human serum and Plasma (citrate).

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Gastrin 1 antigen and a polyclonal rabbit antibody specific to Gastrin 1. After incubation the excess reagents are washed away. pNpp substrate is added and catalyzed by AP to produce a yellow color. The optical density of the yellow coloration at 405 nm is inversely proportional to the amount of Gastrin 1 captured in the plate.

  • Notes

    Gastrins are a family of sequence-related carboxyamidated peptides produced by endocrine G Cells of the antrum mucosa in response to a number of stimuli associated with digestion. Antral distension, partially digested proteins, amino acids, and vagal stimulation resulting from smelling, tasting, chewing or swallowing food all contribute to gastrin release from G Cell storage. In addition, caffeine, alcohol, hypoglycemia, antacids and elevated calcium levels will also stimulate gastrin release. Increased serum gastrin levels are associated with duodenal ulcers, Helicobacter pylori infections, colorectal carcinomas, and other tumors and cancerous lesions. Gastrin is the most potent stimulator of gastric acid secretion.

    Gastrin-I (G17): H2N-Glu-Gly-Pro-Trp-Leu-Glu-Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2

    Gastrin is synthesized as a 101 residue pre-pro-peptide on the rough endoplasmic reticulum, then post-translationally modified by cleavage and alpha-amidation to result in the active forms G34, G17 and G13/14; Big, Little and Mini-Gastrins respectively. Other forms also exist, but are not considered biologically significant. There are two types of G17 and G34, type II is sulfated at the tyrosine12 residue, while type I is not. Both G34 and G17 circulate and contribute to the stimulation of gastric acid secretion, but have different clearance rates. In man, G17 has a circulating half-life of about 9 minutes while G34 has a half-life of about 35 minutes.

    G34 is the major circulating gastrin in fasting serum, but with G17, increases two to three-fold after feeding until both are present in approximately equal amounts. Fasting gastrin concentration is usually less than 30 pmol/l (equivalent to 62.9 pg/ml) while patients with Zollinger-Ellison Syndrome and gastrinomas have levels exceeding 1,000 pg/ml.

    Cross Reactivity

    Compound% Cross Reactivity
    Human Gastrin I (G17-I)100
    Minigastrin (G13-I)74.6
    Rat Gastrin I70.7
    Gastrin II (G17-II, sulfated)9.3
    Cholecystokinin 26-33 (CCK-8)2.67
    Gastrin Tetrapeptide (CCK-4)1.6
    Big Gastrin (G34-I)0.8
    Gastrin Releasing Peptide (GRP)<0.001
  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curev using ab133033

Protocols

References

ab133033 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Gastrin-1 concentration in normal human serum

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Human sera were diluted 4 times by Assay Buffer (serum 25 uL + Assay Buffer 75 uL).
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Submitted Jun 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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