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Abcam’s Human GC Globulin (Vitamin D Binding Protein) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of GC Globulin in plasma, serum, saliva, milk and cell-culture supernatants.
A GC Globulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated GC Globulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of GC Globulin captured in plate.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent M Concentrate||1 x 30ml|
|3X Biotinylated Human GC Globulin (Lyophilized)||1 vial|
|20X Wash Buffer Concentrate||1 x 30ml|
|Chromogen Substrate||1 x 8ml|
|GC Globulin Microplate (12 x 8 well strips)||1 unit|
|GC Globulin Standard||1 vial|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent dilution.|
Representative Standard Curve using ab108853.
ab108853 has not yet been referenced specifically in any publications.