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|Sample type||Average %||Range|
|Serum||104.2||92% - 116%|
|Plasma||102.9||88% - 119%|
|Cell culture media||114.2||96% - 105%|
Abcam's GDF-15 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human GDF-15 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human GDF-15 coated on a 96-well plate. Standards and samples are pipetted into the wells and GDF-15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GDF-15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GDF-15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent||1 x 15ml|
|500X HRP-Streptavidin Concentrate||1 x 200µl|
|Biotinylated anti-Human GDF-15||2 vials|
|GDF-15 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human GDF-15 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab155432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
GDF-15 measured in cell culture supernatants showing quantity (ng) per mL of tested sample. Samples were diluted 20-540 fold.
GDF-15 measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 2-54 fold.
ab155432 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"