Human TNFSF18 / GITRL ELISA Kit (ab113323)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 41 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TNFSF18 / GITRL ELISA Kit
See all TNFSF18/GITRL kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 25 pg/ml -
Range
41 pg/ml - 10000 pg/ml -
Recovery
97 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 87.83 67% - 100% Serum 116.9 108% - 123% Plasma 87.69 67% - 100% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human TNFSF18 / GITRL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNFSF18 / GITRL in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for human TNFSF18 / GITRL coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFSF18 / GITRL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNFSF18 / GITRL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFSF18 / GITRL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human GITR Ligand Item F 2 vials TNFSF18 Ligand Microplate (12 x 8 wells) 1 unit Recombinant Human GITR Ligand Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Cytokine that binds to TNFRSF18/AITR/GITR. Important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues. -
Tissue specificity
Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Activation inducible TNF related ligand
- Activation-inducible TNF-related ligand
- AITR ligand
see all -
Database links
- Entrez Gene: 8995 Human
- Omim: 603898 Human
- SwissProt: Q9UNG2 Human
- Unigene: 248197 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab113323 has not yet been referenced specifically in any publications.