Overview

  • Product nameHuman GITR Ligand ELISA Kit (TNFSF18)
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 25 pg/ml
  • Range
    41 pg/ml - 10000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 87.83 67% - 100%
    Serum 116.9 108% - 123%
    Plasma 87.69 67% - 100%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s GITR Ligand Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GITR Ligand in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human GITR Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and GITR Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GITR Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GITR Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab113323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human GITR Ligand ELISA Kit (TNFSF18) images

  • Representative standard curve using ab113323

Protocols

References for Human GITR Ligand ELISA Kit (TNFSF18) (ab113323)

ab113323 has not yet been referenced specifically in any publications.

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