Overview

  • Product name
    Human Glucokinase ELISA Kit (GCK)
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.4%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1.5 ng/ml
  • Range
    1.563 ng/ml - 50 ng/ml
  • Recovery

    96 %

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Glucokinase (GCK) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human GCK in plasma, serum, and cell culture supernatants.

    A Glucokinase specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Glucokinase specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Glucokinase captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    60X Biotinylated Human Glucokinase Antibody 1 x 100µl
    Chromogen Substrate 1 x 8ml
    Glucokinase Microplate (12 x 8 well strips) 1 unit
    Glucokinase Standard 1 vial
    Sealing Tapes 3 units
    Standard Diluent 1 x 2ml
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.
  • Tissue specificity
    Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.
  • Involvement in disease
    Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) [MIM:125851]; also shortened MODY-2. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    Defects in GCK are the cause of familial hyperinsulinemic hypoglycemia type 3 (HHF3) [MIM:602485]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur.
  • Sequence similarities
    Belongs to the hexokinase family.
  • Information by UniProt
  • Alternative names
    • ATP D hexose 6 phosphotransferase
    • FGQTL3
    • GCK
    • GK
    • GLK
    • Glucokinase
    • Glucokinase (hexokinase 4)
    • hexokinase 4
    • hexokinase D
    • hexokinase D, pancreatic isozyme
    • Hexokinase type IV
    • Hexokinase-4
    • Hexokinase-D
    • HHF3
    • HK IV
    • HK4
    • HKIV
    • HXK4_HUMAN
    • HXKP
    • LGLK
    • Liver Glucokinase
    • MODY2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab125967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab125967

Protocols

References

ab125967 has not yet been referenced specifically in any publications.

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