Human Glucose 6 phosphate isomerase peptide (ab85818)

Overview

Description

  • NatureSynthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab85818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Glucose 6 phosphate isomerase antibody (ab68643)

  • Purity70 - 90% by HPLC.

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • AMF
    • Aurocrine motility factor
    • Autocrine motility factor
    • DKFZp686C13233
    • EC 5.3.1.9
    • G6PI_HUMAN
    • Glucose phosphate isomerase
    • Glucose-6-phosphate isomerase
    • GNPI
    • GPI
    • Gpi1
    • Hexose monophosphate isomerase
    • Hexosephosphate isomerase
    • Neuroleukin
    • NLK
    • Oxoisomerase
    • PGI
    • PHI
    • Phosphoglucose isomerase
    • Phosphohexomutase
    • Phosphohexose isomerase
    • Phosphosaccharomutase
    • SA 36
    • SA-36
    • SA36
    • Sperm antigen 36
    see all
  • FunctionBesides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
  • PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
  • Involvement in diseaseDefects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
  • Sequence similaritiesBelongs to the GPI family.
  • Post-translational
    modifications
    Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
    ISGylated.
  • Cellular localizationCytoplasm. Secreted.
  • Information by UniProt

References for Human Glucose 6 phosphate isomerase peptide (ab85818)

ab85818 has not yet been referenced specifically in any publications.

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