The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-Glucose Transporter GLUT2 antibody (ab95256)
Purity70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Glucose Transporter 2
Glucose Transporter GLUT2
Glucose transporter type 2
Glucose transporter type 2 liver
Glucose transporter, liver/islet
Solute carrier family 2 (facilitated glucose transporter) member 2
Solute carrier family 2 facilitated glucose transporter member 2
Solute carrier family 2, facilitated glucose transporter member 2
FunctionFacilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.
Tissue specificityLiver, insulin-producing beta cell, small intestine and kidney.
Involvement in diseaseFanconi-Bickel syndrome
Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
Post-translational modificationsN-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.