Overview

  • Product nameHuman GM-CSF ELISA Kit
    See all GM-CSF kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    2.05 pg/ml - 500 pg/ml
  • Recovery

    > 75 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101.1 89% - 114%
    Serum 75.43 69% - 83%
    Plasma 74.49 68% - 83%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s GM-CSF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GM-CSF in serum, plasma, and cell culture supernatants

    This assay employs an antibody specific for Human GM-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • Sequence similaritiesBelongs to the GM-CSF family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Colony stimulating factor
    • Colony Stimulating Factor 2
    • Colony stimulating factor 2 (granulocyte-macrophage)
    • Colony-stimulating factor
    • CSF
    • CSF 2
    • CSF2
    • CSF2_HUMAN
    • GM-CSF
    • GMCSF
    • Granulocyte Macrophage Colony Stimulating Factor
    • Granulocyte-macrophage colony-stimulating factor
    • MGC131935
    • MGC138897
    • MGI1GM
    • Molgramostin
    • Pluripoietin-a
    • Sargramostim
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human GM-CSF ELISA Kit images

Protocols

References for Human GM-CSF ELISA Kit (ab100529)

ab100529 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

I have reviewed the data you have kindly sent and I am assuming that the data is for standard curve. The readings seems not good enough because the OD for 0 is comparable to 2.05pg/ml. The OD for "0" pg/m...

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Yes, It is all free however they need to check if the items were paid in original order.

It is just a normal check so there is no need to worry, you will be getting a order confirmation soon.

Our accountsdepartment is holding the order at the moment. The order will be shipped once they authorize it, after checking thepayment etc. Please email mailto:creditcontrol@abcam.com for any order update.

Thanks!

Thank you for contacting us.

To prevent the shortageissue in the future, please advise your customer along with other customers to check all reagents before beginning the experiment and that if there is a volume shortage or any other issue to ...

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Thank you for your cooperation.

I have sent a request to lab for new kits and hoping to get an answer later this evening. I will confirm you the dispatch date.

I hope this info will be helpful.

Thank you for your email.

Regarding ab100562 have you used both kits? If not, then I will send you one replacement kit and one extra vial of HRP-standard. Could you let me know if you still have to use one kit of ab100562?

There are m...

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That's fine!

I will be sending you a replacement kitofab100562.

Could you check the HRP-streptavidin amount in ab100529.Is it OK?

Thank you for your email. I apologize for any inconvenience caused.

The volume 200ul is actually more than what is need for the assay; this extra volume is added to avoid any loss due to evaporation, insufficient centrifuging, etc. Actually on...

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Thank you for your reply.
I confirm that 2.05 pg/ml - 500 pg/ml is the range the kit detects.
Please consult the appropriate literature for the normal concentration for typical concentration of GM-CSF in healthy humans.
A typical standar...

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Thank you for your inquiry.
I am happy to confirm that the range of this kit is typically 2.05 pg/ml - 500 pg/ml as stated on the datasheet.
We also have data on the recovery that can also be found on the datasheet and in the protocol booklet...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"