Human GM-CSF Receptor alpha ELISA Kit (CD116) (ab46086)

Overview

  • Product name
    Human GM-CSF Receptor alpha ELISA Kit (CD116)
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 31 700.2pg/ml 20.4 2.9%
    B 32 378pg/ml 12.8 3.4%
    Inter-assay
    Sample n Mean SD CV%
    A 6 555pg/ml 22.2 4%
    B 6 120.7pg/ml 2.2 1.8%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 16 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Assay time
    3h 45m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s GM-CSF Receptor alpha (CD116) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GM-CSF R alpha in serum, plasma, and cell culture supernatants.

    A monoclonal antibody specific for GM-CSF R alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known GM-CSF R alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for GM-CSF R alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of GM-CSF R alpha present in the samples.

    This kit will recognize both endogenous and recombinant Human GM-CSF R alpha.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-GM-CSF R alpha Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    Control Silver 2 vials 4 vials
    GM-CSF Receptor alpha Microplate (12 x 8 well strips) 1 unit 2 units
    GM-CSF Receptor alpha Standard (Lyophilized) Yellow 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas
  • Relevance
    GM-CSF Receptor alpha is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble.
  • Cellular localization
    Cell membrane; single-pass type I membrane protein. Isoform 3, isoform 4, isoform 6: Secreted protein
  • Alternative names
    • CD116
    • CD116 antigen
    • CDw116
    • Colony stimulating factor 2 receptor alpha low affinity (granulocyte macrophage)
    • CSF2R
    • CSF2RAX
    • CSF2RAY
    • CSF2RX
    • CSF2RY
    • GM CSF R alpha
    • GMCSFR
    • GMR
    • Granulocyte macrophage colony stimulating factor receptor alpha chain
    • MGC3848
    • MGC4838
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab46086

Protocols

References

ab46086 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry. The information available concerning this kit suggests that it will still recognize the GM-CSF Receptor when bound to the related cytokine (GM-CSF). This has not been verified by our lab. I hope this information is...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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