Human GM-CSF Receptor alpha ELISA Kit (CD116) (ab46086)


  • Product name
    Human GM-CSF Receptor alpha ELISA Kit (CD116)
  • Detection method
  • Precision
    Sample n Mean SD CV%
    A 31 700.2pg/ml 20.4 2.9%
    B 32 378pg/ml 12.8 3.4%
    Sample n Mean SD CV%
    A 6 555pg/ml 22.2 4%
    B 6 120.7pg/ml 2.2 1.8%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 16 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Assay time
    3h 45m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s GM-CSF Receptor alpha (CD116) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GM-CSF R alpha in serum, plasma, and cell culture supernatants.

    A monoclonal antibody specific for GM-CSF R alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known GM-CSF R alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for GM-CSF R alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of GM-CSF R alpha present in the samples.

    This kit will recognize both endogenous and recombinant Human GM-CSF R alpha.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-GM-CSF R alpha Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    Control Silver 2 vials 4 vials
    GM-CSF Receptor alpha Microplate (12 x 8 well strips) 1 unit 2 units
    GM-CSF Receptor alpha Standard (Lyophilized) Yellow 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas
  • Relevance
    GM-CSF Receptor alpha is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble.
  • Cellular localization
    Cell membrane; single-pass type I membrane protein. Isoform 3, isoform 4, isoform 6: Secreted protein
  • Alternative names
    • CD116
    • CD116 antigen
    • CDw116
    • Colony stimulating factor 2 receptor alpha low affinity (granulocyte macrophage)
    • CSF2R
    • CSF2RAX
    • CSF2RAY
    • CSF2RX
    • CSF2RY
    • GM CSF R alpha
    • GMCSFR
    • GMR
    • Granulocyte macrophage colony stimulating factor receptor alpha chain
    • MGC3848
    • MGC4838
    see all
  • Database links


Our Abpromise guarantee covers the use of ab46086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human GM-CSF Receptor alpha ELISA Kit (CD116) images

  • Representative Standard Curve using ab46086


References for Human GM-CSF Receptor alpha ELISA Kit (CD116) (ab46086)

ab46086 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The information available concerning this kit suggests that it will still recognize the GM-CSF Receptor when bound to the related cytokine (GM-CSF). This has not been verified by our lab. I hope this information is...

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