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|Sample type||Average %||Range|
|Serum||73||31.25pg/ml - 1000pg/ml|
Abcam’s Granzyme B (CTLA-1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Granzyme B in Human serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific Granzyme B has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Granzyme B concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Granzyme B are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Granzyme B present in the samples.
This kit will recognize both endogenous and recombinant Human Granzyme B.
|Components||Identifier||1 x 96 tests||2 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|200X Wash Buffer||White||1 x 10ml||2 x 10ml|
|Biotinylated Antibody Diluent||Red||1 x 7.5ml||1 x 13ml|
|Biotinylated anti-Granzyme B||Red||1 x 400µl||2 x 400µl|
|Chromogen TMB Substrate Solution||1 x 11ml||1 x 24ml|
|Granzyme B Microplate (12 x 8 well strips)||1 unit||2 units|
|Granzyme B Standard (Lyophilized)||Yellow||2 vials||4 vials|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|Stop Reagent||Black||1 x 11ml||2 x 11ml|
|Streptavidin-HRP||2 x 5µl||4 x 5µl|
Our Abpromise guarantee covers the use of ab46142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Hu Granzyme B (CTLA-1) measured in biologicals showing quantity (pg) per mL of tested sample
Representative Standard Curve using ab46142