Overview

  • Product nameHuman GRO alpha ELISA Kit (CXCL1)
    See all GRO alpha kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    2 pg/ml
  • Range
    2 pg/ml - 1000 pg/ml
  • Recovery

    > 99 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 112.2 101% - 122%
    Serum 130.4 119% - 145%
    Plasma 113.5 85% - 140%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s GRO alpha (CXCL1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme designed for the quantitative measurement of Human GRO alpha in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human GRO alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and GRO alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GRO alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GRO alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceThe GRO gene was originally identified by subtractive hybridization studies between normal and tumorigenic Chinese hamster embryo fibroblasts. The hamster cDNA was cloned and used as a probe for cloning of the human GRO cDNA. The GROalpha gene initially cloned from T24 cells and the gene in melanoma cells encoding melanoma growth stimulating protein (MGSA) are identical. Human cells contain three closely related, but distinct GRO genes: GRO alpha, GRO beta, and GRO gamma. GRO beta and GRO gamma share 93% and 82% identity, respectively, with GRO alpha at the nucleotide level. GROs are members of the chemokine alpha family that is characterized by the separation with one amino acid of the first two cysteine residues, C-X-C, in the amino acid sequence. The GRO gene has been mapped to chromosome 4q21. In normal cells, human mRNA GRO expression is found in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, GRO mRNA has been detected in mammary fibroblasts, mammary epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. Characterization of the GROalpha receptor indicates the presence of low and high affinity receptors on human neutrophils.
  • Cellular localizationSecreted
  • Alternative names
    • C-X-C motif chemokine 1
    • chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
    • fibroblast secretory protein
    • FSP
    • GRO
    • GRO protein, alpha
    • GRO-alpha(1-73)
    • GRO1
    • GRO1 oncogene
    • GRO1 oncogene (melanoma growth stimulating activity, alpha)
    • GRO1 oncogene (melanoma growth-stimulating activity)
    • GROa
    • growth-regulated alpha protein
    • Keratinocyte-derived chemokine, mouse, homolog of
    • Melanoma growth stimulatory activity
    • melanoma growth stimulatory activity alpha
    • MGSA
    • MGSA a
    • MGSA alpha
    • NAP 3
    • Neutrophil-activating protein 3
    • SCYB1
    • Small inducible cytokine subfamily B, member 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human GRO alpha ELISA Kit (CXCL1) images

  • Representative standard curve using ab100530

Protocols

References for Human GRO alpha ELISA Kit (CXCL1) (ab100530)

ab100530 has not yet been referenced specifically in any publications.

Product Wall

CXCL1 - GROa secretion by human macrophages & DC

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CXCL1 human macrophage & DC secretion in culture for 3 and 7 days, isolated by negative selection.
The assay is easy to use and takes about 5.30hr.
Dilutions may be necessary.
Good specificity as shown by CXCL1 secretion by M1 & M2c-IL10 macrophages subtyes.
M0 = MCSF M1 = MCSF+IFNg M2a = MCSF+IL4 M2c = MCSF+TGFb or MCSF+IL10 or MCSF+Dexamethasone DC = GMCSF+IL4.
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Submitted May 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"