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|Sample type||Average %||Range|
|Cell culture supernatant||104||100% - 109%|
|Milk||83||81% - 84%|
|Urine||99||97% - 105%|
|Serum||98||92% - 102%|
|Heparin Plasma||97||89% - 106%|
|EDTA Plasma||91||78% - 102%|
|Citrate Plasma||99||90% - 114%|
Abcam’s Growth Hormone (GH) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Growth Hormone (GH) protein in human serum, plasma and cell culture supernatant.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Growth Hormone (also known as GH, GH1, Somatotropin and Pituitary growth hormone) is a circulating hormone that plays an important role in somatic growth control. Growth Hormone binds to the Growth Hormone Receptor present in a variety of tissues and induces signaling cascades. Growth Hormone secretion is controlled positively and negatively by other hormones, including Ghrelin and Somatostatin. Overproduction of Growth Hormone can result in gigantism whereas deficiency can contribute to dwarfism.
|Components||1 x 96 tests|
|10X Growth Hormone Capture Antibody||1 x 600µl|
|10X Growth Hormone Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|ab221824 - Antibody Diluent 4BI||1 x 6ml|
|Growth Hormone Human Lyophilized Recombinant Protein||2 vials|
|Plate Seals||1 unit|
|Sample Diluent NS (ab193972)||1 x 50ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab190811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Example of Growth Hormone standard curve.
Serum from 10 apparently healthy male donors was measured in duplicate. The mean Growth Hormone concentration was determined to be 365 pg/mL with a range of 15-1,779 pg/mL in male donors.
The concentrations of Growth Hormone were measured in duplicate and interpolated from the Growth Hormone standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Growth Hormone concentration was determined to be 242 pg/mL in serum and 216 pg/mL in plasma.
Background-subtracted data values (mean +/- SD, n =2) are graphed.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"