The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityThis peptide has been shown to block the detection of a 179kDa band by anti-HDPTP (ab12553) in Western Blot.
Blocking - Blocking peptide for Anti-HDPTP antibody (ab12553)
Additional notesThis peptide has been shown to block the detection of a 179kDa band by anti-HDPTP (ab12553) in Western Blot.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
His domain containing protein tyrosine phosphatase
His domain protein tyrosine phosphatase
His domain-containing protein tyrosine phosphatase
Protein tyrosine phosphatase non receptor type 23
Protein tyrosine phosphatase TD 14
Protein tyrosine phosphatase TD14
Tyrosine protein phosphatase non receptor type 23
Tyrosine-protein phosphatase non-receptor type 23
FunctionMay act as a negative regulator of Ras-mediated mitogenic activity. Plays a role in ciliogenesis.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 BRO1 domain. Contains 2 TPR repeats. Contains 1 tyrosine-protein phosphatase domain.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.