• Product name
    Human Heat Shock Protein 27 ELISA Kit (HSP27)
    See all Heat Shock Protein 27 (HSP27) kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall 4.6%
    Sample n Mean SD CV%
    Overall 7.3%
  • Sample type
    Milk, Serum, Plasma, Cell culture extracts, Tissue
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    > 0.3 ng/ml
  • Range
    0.313 ng/ml - 80 ng/ml
  • Recovery

    97 %

  • Assay time
    5h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Heat Shock Protein 27 (HSP27) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP27 levels in cell culture samples, milk, serum, plasma and tissue extract.

    A HSP27 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP27 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP27 captured in plate.

    Get results in 90 minutes with Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab193757) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Relevance
    Involved in stress resistance and actin organization. Interacts with TGFB1I1. Associates with alpha- and beta-tubulin, microtubules and CRYAB. Interacts with HSPB8 and HSPBAP1.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasm; cytoskeleton; spindle. Note: Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Alternative names
    • HSPB1
    • 28 kDa heat shock protein
    • CMT2F
    • estrogen regulated 24 kDa protein
    • Estrogen-regulated 24 kDa protein
    • Heat shock 27 kDa protein
    • heat shock 27kDa protein 1
    • heat shock protein beta 1
    • Heat shock protein beta-1
    • HMN2B
    • HS.76067
    • HSP 27
    • Hsp25
    • HSP27
    • HSP28
    • HspB1
    • SRP27
    • stress responsive protein 27
    • Stress-responsive protein 27
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab108862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human Heat Shock Protein 27 ELISA Kit (HSP27) images

  • Representative Standard Curve using ab108862.


References for Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab108862)

This product has been referenced in:
  • White NM  et al. Quantitative proteomic analysis reveals potential diagnostic markers and pathways involved in pathogenesis of renal cell carcinoma. Oncotarget 5:506-18 (2014). Read more (PubMed: 24504108) »
  • Enomoto M  et al. Newborn rat response to single vs. combined cGMP-dependent pulmonary vasodilators. Am J Physiol Lung Cell Mol Physiol 306:L207-15 (2014). WB . Read more (PubMed: 24242011) »

See all 3 Publications for this product

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