Overview

  • Product name
    Human Heat Shock Protein 27 ELISA Kit (HSP27)
    See all Heat Shock Protein 27 (HSP27) kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.3%
  • Sample type
    Milk, Serum, Plasma, Cell culture extracts, Tissue
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    > 0.3 ng/ml
  • Range
    0.313 ng/ml - 80 ng/ml
  • Recovery

    97 %

  • Assay time
    5h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Heat Shock Protein 27 (HSP27) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP27 levels in cell culture samples, milk, serum, plasma and tissue extract.


    A HSP27 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP27 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP27 captured in plate.


    Get results in 90 minutes with Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab193757) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108862.

Protocols

References

This product has been referenced in:
  • White NM  et al. Quantitative proteomic analysis reveals potential diagnostic markers and pathways involved in pathogenesis of renal cell carcinoma. Oncotarget 5:506-18 (2014). Read more (PubMed: 24504108) »
  • Enomoto M  et al. Newborn rat response to single vs. combined cGMP-dependent pulmonary vasodilators. Am J Physiol Lung Cell Mol Physiol 306:L207-15 (2014). WB . Read more (PubMed: 24242011) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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