|Sample type||Average %||Range|
|Cell culture supernatant||133.1||123% - 151%|
|Serum||126.9||117% - 134%|
|Plasma||119.8||105% - 142%|
Abcam’s Heat Shock Protein 27 (HSP27) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HSP27 in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human Heat Shock Protein 27 coated on a 96-well plate. Standards and samples are pipetted into the wells and Heat Shock Protein 27 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Heat Shock Protein 27 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Heat Shock Protein 27 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|300X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|Biotinylated anti-Human Heat Shock Protein 27||2 vials|
|Heat Shock Protein 27 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human Heat Shock Protein 27 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab113334 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab113334 has not yet been referenced specifically in any publications.