Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab113334)

Overview

  • Product name
    Human Heat Shock Protein 27 ELISA Kit (HSP27)
    See all Heat Shock Protein 27 (HSP27) kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.15 ng/ml
  • Range
    0.205 ng/ml - 50 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 133.1 123% - 151%
    Serum 126.9 117% - 134%
    Plasma 119.8 105% - 142%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Heat Shock Protein 27 (HSP27) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HSP27 in serum, plasma, cell culture supernatants.


    This assay employs an antibody specific for Human Heat Shock Protein 27 coated on a 96-well plate. Standards and samples are pipetted into the wells and Heat Shock Protein 27 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Heat Shock Protein 27 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Heat Shock Protein 27 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab193757) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab113334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab113334

Protocols

References

ab113334 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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