Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab108861)

Overview

  • Product nameHuman Heat Shock Protein 47 ELISA Kit (SERPINH1)
    See all Hsp 47 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 2.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.6%
  • Tests
    1 x 96 well plate
  • Sample type
    Milk, Serum, Plasma, Tissue, Cell Lysate
  • Assay typeSandwich (quantitative)
  • Sensitivity
    > 0.9 ng/ml
  • Range
    1.563 ng/ml - 50 ng/ml
  • Recovery

    96 %

  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Heat Shock Protein 47 (SERPINH1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP47 levels in Human cell culture lysates, milk, serum, plasma and tissue extract.

    A HSP47 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP47 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP47 captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionBinds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • Involvement in diseaseDefects in SERPINH1 are the cause of osteogenesis imperfecta type 10 (OI10) [MIM:613848]. A connective tissue disorder characterized by bone fragility, low bone mass, bowing of limbs due to multiple fractures, short limb dwarfism and blue sclerae.
  • Sequence similaritiesBelongs to the serpin family.
  • Cellular localizationEndoplasmic reticulum lumen.
  • Information by UniProt
  • Alternative names
    • 47 kDa heat shock protein
    • Arsenic-transactivated protein 3
    • AsTP3
    • Cell proliferation-inducing gene 14 protein
    • Collagen-binding protein
    • Collagen-binding protein 1
    • Colligin
    • Rheumatoid arthritis-related antigen RA-A47
    • SERPH_HUMAN
    • Serpin H1
    • Serpin Peptidase Inhibitor
    • SERPINH1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human Heat Shock Protein 47 ELISA Kit (SERPINH1) images

  • Representative Standard Curve using ab108861.

Protocols

References for Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab108861)

This product has been referenced in:
  • Fang JY  et al. Skin aging caused by intrinsic or extrinsic processes characterized with functional proteomics. Proteomics 16:2718-2731 (2016). Read more (PubMed: 27459910) »

See 1 Publication for this product

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