Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab108861)

Overview

  • Product name
    Human Heat Shock Protein 47 ELISA Kit (SERPINH1)
    See all Hsp 47 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 2.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.6%
  • Sample type
    Milk, Serum, Plasma, Tissue, Cell Lysate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    > 0.9 ng/ml
  • Range
    1.563 ng/ml - 50 ng/ml
  • Recovery

    96 %

  • Assay time
    5h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Heat Shock Protein 47 (SERPINH1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP47 levels in Human cell culture lysates, milk, serum, plasma and tissue extract.


    A HSP47 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP47 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP47 captured in plate.


    Get results in 90 minutes with Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab202407) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Function
    Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • Involvement in disease
    Defects in SERPINH1 are the cause of osteogenesis imperfecta type 10 (OI10) [MIM:613848]. A connective tissue disorder characterized by bone fragility, low bone mass, bowing of limbs due to multiple fractures, short limb dwarfism and blue sclerae.
  • Sequence similarities
    Belongs to the serpin family.
  • Cellular localization
    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Alternative names
    • 47 kDa heat shock protein
    • Arsenic-transactivated protein 3
    • AsTP3
    • Cell proliferation-inducing gene 14 protein
    • Collagen-binding protein
    • Collagen-binding protein 1
    • Colligin
    • Rheumatoid arthritis-related antigen RA-A47
    • SERPH_HUMAN
    • Serpin H1
    • Serpin Peptidase Inhibitor
    • SERPINH1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab108861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human Heat Shock Protein 47 ELISA Kit (SERPINH1) images

  • Representative Standard Curve using ab108861.

Protocols

References for Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab108861)

This product has been referenced in:
  • Fang JY  et al. Skin aging caused by intrinsic or extrinsic processes characterized with functional proteomics. Proteomics 16:2718-2731 (2016). Read more (PubMed: 27459910) »

See 1 Publication for this product

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