Human Anti-Helicobacter pylori IgA ELISA Kit (ab108762)

Overview

  • Product nameHuman Anti-Helicobacter pylori IgA ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Standard B 12 7.2%
    Standard C 16 5.9%
    Inter-assay
    Sample n Mean SD CV%
    Standard B 3 9.6%
    Standard C 3 6.7%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Sensitivity
    < 5 U/ml
  • Range
    5 U/ml - 150 U/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Helicobacter pylori IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgA class antibodies against Helicobacter pylori in Human serum and plasma.

     

    A 96-well plate has been precoated with Helicobacter pylori antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Helicobacter pylori-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Helicobacter pylori IgA sample captured in plate.

  • Tested applicationsSuitable for: Indirect ELISAmore details
  • PlatformMicroplate

Properties

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab108762 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent dilution.

    Human Anti-Helicobacter pylori IgA ELISA Kit images

    • Representative Standard Curve using ab108762

    Protocols

    References for Human Anti-Helicobacter pylori IgA ELISA Kit (ab108762)

    ab108762 has not yet been referenced specifically in any publications.

    Product Wall

    ab108736 incorporates highly purified proteins associated with CagA genes (120 KD) and VaCA genes (87 KD)as well as urease-antigens.

    ab108762 incorporates recombinant CagA and recombinant urease-antigens.

    To the best of our knowledge, there is not cross reactivity with IgG.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"