Human Anti-Helicobacter pylori IgG ELISA Kit (ab108736)

Overview

  • Product name
    Human Anti-Helicobacter pylori IgG ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 19 6.1%
    Pos.Serum 19 7%
    Inter-assay
    Sample n Mean SD CV%
    Pos.Serum 13 7.2%
    Pos.Serum 13 3%
  • Sample type
    Serum, Plasma
  • Assay type
    Indirect
  • Sensitivity
    3 U/ml
  • Range
    3 U/ml - 150 U/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Helicobacter pylori IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Helicobacter pylori in Human serum and plasma.

     

    A 96-well plate has been precoated with Helicobacter pylori antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Helicobacter pylori-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Helicobacter pylori IgG sample captured in plate.

  • Tested applications
    Suitable for: Indirect ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108736

Protocols

References

ab108736 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

ab108736 incorporates highly purified proteins associated with CagA genes (120 KD) and VaCA genes (87 KD)as well as urease-antigens.

ab108762 incorporates recombinant CagA and recombinant urease-antigens.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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