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Abcam’s anti-Helicobacter pylori IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Helicobacter pylori in Human serum and plasma.
A 96-well plate has been precoated with Helicobacter pylori antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Helicobacter pylori-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Helicobacter pylori IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|Helicobacter pylori (IgG) Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Helicobacter pylori anti-IgG HRP conjugate||Blue, Black cap||1 x 20ml|
|Helicobacter pylori IgG Standard A – 0 U/mL||Blue Cap||1 x 2ml|
|Helicobacter pylori IgG Standard B – 15 U/mL||Green Cap||1 x 2ml|
|Helicobacter pylori IgG Standard C – 75 U/mL||Yellow cap||1 x 2ml|
|Helicobacter pylori IgG Standard D – 150 U/mL||Red cap||1 x 2ml|
|IgG Sample Diluent||Yellow, White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
ab108736 has not yet been referenced specifically in any publications.