• Product name
    Human Hemopexin ELISA Kit (HPX)
    See all Hemopexin kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall 4.9%
    Sample n Mean SD CV%
    Overall 7.4%
  • Sample type
    Milk, Serum, Plasma
  • Assay type
  • Sensitivity
    > 0.7 µg/ml
  • Range
    0.078 µg/ml - 5 µg/ml
  • Recovery

    98 %

  • Assay time
    2h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Hemopexin (HPX) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of hemopexin levels in plasma and serum.

    A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Hemopexin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Hemopexin captured in plate.

    Get results in 90 minutes with Human Hemopexin ELISA Kit (ab171576) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform


  • Function
    Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Sequence similarities
    Belongs to the hemopexin family.
    Contains 5 hemopexin-like domains.
  • Post-translational
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • Beta 1B glycoprotein
    • Beta-1B-glycoprotein
    • FLJ56652
    • HEMO
    • Hemopexin
    • Hpx
    • HX
    see all
  • Database links


Our Abpromise guarantee covers the use of ab108859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Human Hemopexin ELISA Kit (HPX) images

  • Hemopexin measured in various biological fluids showing quantity (μg) per mL of tested sample

  • Representative Standard Curve using ab108859.


References for Human Hemopexin ELISA Kit (HPX) (ab108859)

This product has been referenced in:
  • Evans C  et al. Impairment of neutrophil oxidative burst in children with sickle cell disease is associated with heme oxygenase-1. Haematologica N/A:N/A (2015). Read more (PubMed: 26315932) »
  • Insenser M  et al. Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism: a nontargeted proteomic study. PLoS One 9:e100902 (2014). Read more (PubMed: 24979072) »

See all 2 Publications for this product

Product Wall

Hemopexin (HPX) Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Sample: EDTA human plasma stored at -80ºC
Amount of Sample: 3 l
Type of Kit Used: ELISA
Manufacturer instructions were followed.
Additional data
We diluted 3 l of plasma in 1200 l of 1XDiluent N (1:400)
Washings were done on a Microplate Washer machine with 300 l of wash buffer.
The standards data generated an excellent curve.
All samples assayed showed data inside the curve.

Abcam user community

Verified customer

Submitted Apr 08 2015

Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit

Read More

Thank you for your enquiry.

Sorry for the confusion here. A large excess of Standard is provided in this ELISA kit. To properly dilute the standard, just reconstitute the 3.5ug Standard you received in 0.7ml of Diluent to produce a 5ug/ml Sta...

Read More

Abreview for Hemopexin Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)

Sample: Human Serum
Amount of Sample: 2 ul µg
Type of Kit Used: ELISA
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: We diluted 2ul of plasma with 800 ul of Diluent (1:400).
We washed the plate 6 times with a machine.
The standards data generated an excelent curve with a fitting error of
All samples assayed showed data inside the curve.

Dr. Rafael Montes Nieto

Verified customer

Submitted Mar 26 2012

Thank you for your phone call. Please find testing data for ab108859 attached. I hope this helps, please let me know if you need any additional information or assistance.


Sign up