Overview

  • Product nameHuman Hemopexin ELISA Kit (HPX)
    See all Hemopexin kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.3%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 6 ng/ml
  • Range
    6.25 ng/ml - 400 ng/ml
  • Recovery

    98 %

  • Assay time
    3h 30m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Hemopexin (HPX) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Hemopexin levels in Human cell culture supernatants, milk, saliva and urine.


    A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Hemopexin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Hemopexin captured in plate.


    For higher sensitivity in only 90 minutes, we recommend Human Hemopexin ELISA Kit (ab171576) from our SimpleStep ELISA® range.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionBinds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Sequence similaritiesBelongs to the hemopexin family.
    Contains 5 hemopexin-like domains.
  • Post-translational
    modifications
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Beta 1B glycoprotein
    • Beta-1B-glycoprotein
    • FLJ56652
    • HEMO
    • HEMO_HUMAN
    • Hemopexin
    • Hpx
    • HX
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab108860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human Hemopexin ELISA Kit (HPX) images

  • Hemopexin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human saliva diluted 60-120 fold. Human milk diluted 300-600 fold. Human urine diluted 10 fold - below detection. Cell supernatants tested neat.

  • Representative Standard Curve using ab108860.

Protocols

References for Human Hemopexin ELISA Kit (HPX) (ab108860)

This product has been referenced in:
  • Zager RA  et al. Renal cortical hemopexin accumulation in response to acute kidney injury. Am J Physiol Renal Physiol 303:F1460-72 (2012). Sandwich ELISA . Read more (PubMed: 22993068) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab108860.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"