Abcam’s anti-Herpes simplex virus Type 2 (HSV2) IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Herpes simplex virus Type 2 in Human serum and plasma.
A 96-well plate has been precoated with Herpes simplex virus Type 2 antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Herpes simplex virus Type 2-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Herpes simplex virus Type 2 IgM sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|Herpes simplex virus Type 2 (IgM) Coated Microplate (12 x 8 wells)||1 unit|
|Herpes simplex virus Type 2 anti-IgM HRP Conjugate||Red , Black cap||1 x 20ml|
|Herpes simplex virus Type 2 IgM Cut-off Control||Yellow , Green cap||1 x 3ml|
|Herpes simplex virus Type 2 IgM Negative Control||Yellow , Blue cap||1 x 2ml|
|Herpes simplex virus Type 2 IgM Positive Control||Yellow , Red cap||1 x 2ml|
|IgM Sample Diluent||Green , White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab108740 has not yet been referenced specifically in any publications.