The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
3' 5' exonuclease ERI 1
3' 5' exonuclease ERI1
3' histone mRNA exonuclease 1
3''-5'' exonuclease eri1
3''-5'' exoribonuclease 1
Eri 1 homolog
Histone mRNA 3' end specific exonuclease
Histone mRNA 3' end specific exoribonuclease
Histone mRNA 3''-end-specific exoribonuclease
Histone mRNA 3''-exonuclease 1
Three prime histone mRNA exonuclease 1
FunctionRNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
Sequence similaritiesContains 1 exonuclease domain. Contains 1 SAP domain.
DomainThe SAP domain is necessary for binding to the stem-loop structure of histone mRNAs and to form the ternary complex with SLBP, but not for 3'-end histone mRNA exonuclease activity.