Overview

  • Product nameHuman HGF ELISA Kit
    See all HGF kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 3 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    > 98 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.35 89% - 108%
    Serum 108.28 96% - 120%
    Plasma 107.56 95% - 119%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s HGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HGF in serum, plasma (EDTA and citrate), and cell culture supernatants.

    This assay employs an antibody specific for Human HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionPotent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • Involvement in diseaseDeafness, autosomal recessive, 39
  • Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Alternative names
    • DFNB39
    • F TCF
    • Fibroblast derived tumor cytotoxic factor
    • Hepatocyte growth factor
    • Hepatocyte growth factor (hepapoietin A; scatter factor)
    • Hepatocyte growth factor beta chain
    • Hepatocyte growth factor precursor
    • Hepatopoietin A
    • Hepatopoietin-A
    • Hgf
    • HGF_HUMAN
    • HGFB
    • HPTA
    • Lung fibroblast derived mitogen
    • OTTHUMP00000161349
    • OTTHUMP00000206710
    • OTTHUMP00000206711
    • OTTHUMP00000206712
    • OTTHUMP00000206713
    • OTTHUMP00000206730
    • Scatter factor
    • SF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human HGF ELISA Kit images

  • HuHGF measured in biological fluids showing quantity (pg) per mL of tested sample.

  • Representative standard curve using ab100534

  • Representative standard curve using ab100534

Protocols

References for Human HGF ELISA Kit (ab100534)

This product has been referenced in:
  • Nishida S  et al. Prostate cancer stem-like cells/cancer-initiating cells have an autocrine system of hepatocyte growth factor. Cancer Sci 104:431-6 (2013). Human . Read more (PubMed: 23320511) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry. I have answered your questions below:

Does this kit detect total HGF - the single chain precursor of HGF (scHGF) and the activated heterodimer as well?

The exact form of HGF recognized has not been tested, ...

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I am sorry for the delay in getting back to you. I now have further data to share with you from the HGF Human ELISA Kit (ab100534).

Please find attached an excel spreadsheet. In the first sheet there is the data collected from two subsequent a...

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I am sorry for the delay in getting back to you. I now have further information to share with you in relation the variation seem by your customer when using the HGF Human ELISA Kit (ab100534).

The source of this kti ahve had a look at the resu...

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Thank you for contacting us.

The reconstituted standard is stable for ˜1 week if stored frozen (-20°C - -80°C). Please ensure avoiding multiple freeze – thaw cycles. We do provide 2 vials of the standard recombinant protein to m...

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Thank you for confirming that. xxxx is currently not in the office but I can help with this case.

I have now added the replacement kit to the order number xxxxxx. Please be aware that the kit may be moved from this order before shipping to be...

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Thank you for your reply.

It can't be that the buffer has the same consistency as glycerol!As we do not have separatecomponents, I would like to offer your customer a FOCR instead.

Please let me know if this is acceptable.

Thank you for sending in the data. The standard curve does still contain strong linearity when including the higher concentrated standards, 222.2 pg/ml and 666.7 pg/ml. The highest standard (2000 pg/ml) did give a reading that affects the linearity dow...

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Thank you for contacting us.

260 uL of Assay Diluent A or 1x Assay Diluent B should be added to all of the vials except the first one. The first vial should contain 390 uL of the Standard that was prepared in 600 uL of Assay Diluent A or B. T...

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It is difficult to recommend a specific speed for a "gentle shaking". The plate should just bemoving to get a homogenous solution and not too fast to avoid a spillage.I hope this helps.

Thank you for contacting us.

Both mouse and human HGF ELISA kits will detect total HGF.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"