Overview

Description

  • NatureSynthetic
  • Amino Acid Sequence
    • AccessionP0C0S5
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab11681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Histone H2A.Z antibody - ChIP Grade (ab4174)

  • FormLiquid
  • Additional notesSynthetic peptide: C-SLIGKKGQQKT, corresponding to amino acids 116-126 of Human H2A.Z.

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • H2A histone family member Z
    • H2A.z
    • H2A/z
    • H2afz
    • H2AZ
    • H2AZ_HUMAN
    • Histone H2A.Z
    • MGC117173
    see all
  • FunctionVariant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
  • Sequence similaritiesBelongs to the histone H2A family.
  • Post-translational
    modifications
    Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
    Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
    Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
    Not phosphorylated.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt

References for Human Histone H2A.Z peptide (ab11681)

This product has been referenced in:
  • Kafer GR  et al. In Vitro Manipulation of Mammalian Preimplantation Embryos Can Alter Transcript Abundance of Histone Variants and Associated Factors. Cell Reprogram : (2011). WB . Read more (PubMed: 21827322) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"