Human Histone H2B (mono methyl K5) peptide (ab13211)

Overview

  • Product name
    Human Histone H2B (mono methyl K5) peptide

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Species
      Human
    • Modifications
      mono methyl K5

Specifications

Our Abpromise guarantee covers the use of ab13211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

General Info

  • Alternative names
    • H2B K
    • H2B type 12
    • H2B/b
    • H2B/c
    • H2B/d
    • H2B/e
    • H2B/f
    • H2B/j
    • H2B/n
    • H2B/q
    • H2B/r
    • H2B/s
    • HIRA-interacting protein 1
    • HIRA-interacting protein 2
    • Histone H2B type 1-B
    • Histone H2B type 1-C/E/F/G/I
    • Histone H2B type 1-D
    • Histone H2B type 1-H
    • Histone H2B type 1-J
    • Histone H2B type 1-K
    • Histone H2B type 1-L
    • Histone H2B type 1-M
    • Histone H2B type 1-N
    • Histone H2B type 1-O
    • Histone H2B type 2-E
    • Histone H2B type 2-F
    • Histone H2B type 3-B
    • Histone H2B type F-S
    • Histone H2B-GL105
    • Histone H2B.1
    • Histone H2B.1 A
    • Histone H2B.1 B
    • Histone H2B.2
    • Histone H2B.a
    • Histone H2B.b
    • Histone H2B.c
    • Histone H2B.d
    • Histone H2B.e
    • Histone H2B.f
    • Histone H2B.g
    • Histone H2B.h
    • Histone H2B.j
    • Histone H2B.k
    • Histone H2B.l
    • Histone H2B.n
    • Histone H2B.r
    • Histone H2B.s
    see all
  • Relevance
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
  • Cellular localization
    Nuclear

References

ab13211 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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