Our Abpromise guarantee covers the use of ab1783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Information available upon request.
Rabbit polyclonal to Histone H3 mono methyl K36 (ab9048) on Calf Thymus Histones (2 µg of Histone prep per lane).
Lane 1: ab9048 at 1/1000
Lane 2: ab9048 at 1/1000 + 1 µg/ml Mono methyl K36 peptide (ab1783)
Lane 3: ab9048 at 1/1000 + 1 µg/ml Di methyl K36 peptide (ab1784)
Lane 4: ab9048 at 1/1000 + 1 µg/ml Tri methyl K36 peptide (ab1785)
Secondary antibody : Goat anti-rabbit (HRP) at 1/2000 (ab6721)