Human Histone H3 (tri methyl K4) peptide (ab1342)

Overview

Description

  • NatureSynthetic
  • Amino Acid Sequence
    • SpeciesHuman
    • Modificationstri methyl K4

Specifications

Our Abpromise guarantee covers the use of ab1342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Dot Blot

    Blocking - Blocking peptide for Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580), Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade (ab12209)

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

General Info

  • Alternative names
    • H3 histone family, member J
    • FLJ92264
    • H3 histone family, member A
    • H3 histone family, member B
    • H3 histone family, member C
    • H3 histone family, member D
    • H3 histone family, member F
    • H3 histone family, member H
    • H3 histone family, member I
    • H3 histone family, member K
    • H3 histone family, member L
    • H3 histone, family 3A
    • H3.3A
    • H3/a
    • H3/b
    • H3/c
    • H3/d
    • h3/f
    • H3/h
    • H3/i
    • H3/j
    • H3/k
    • H3/l
    • H31_HUMAN
    • H3F1K
    • H3F3
    • H3FA
    • H3FB
    • H3FC
    • H3FD
    • H3FF
    • H3FH
    • H3FI
    • H3FJ
    • H3FK
    • H3FL
    • HIST1H3A
    • HIST1H3B
    • HIST1H3C
    • HIST1H3D
    • HIST1H3E
    • HIST1H3F
    • HIST1H3G
    • HIST1H3H
    • HIST1H3I
    • HIST1H3J
    • HIST3H3
    • Histone 1, H3a
    • Histone 1, H3b
    • Histone 1, H3c
    • Histone 1, H3d
    • Histone 1, H3e
    • Histone 1, H3f
    • Histone 1, H3g
    • Histone 1, H3h
    • Histone 1, H3i
    • Histone 1, H3j
    • Histone cluster 1, H3a
    • Histone cluster 1, H3b
    • Histone cluster 1, H3c
    • Histone cluster 1, H3d
    • Histone cluster 1, H3e
    • Histone cluster 1, H3f
    • Histone cluster 1, H3g
    • Histone cluster 1, H3i
    • Histone cluster 1, H3j
    • Histone H 3
    • Histone H3.1
    • Histone H3.2
    • Histone H3/a
    • Histone H3/b
    • Histone H3/c
    • Histone H3/d
    • Histone H3/f
    • Histone H3/h
    • Histone H3/i
    • Histone H3/j
    • Histone H3/k
    • Histone H3/l
    • Histone H3/m
    • Histone H3/o
    see all
  • FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similaritiesBelongs to the histone H3 family.
  • Developmental stageExpressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • Post-translational
    modifications
    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt

Human Histone H3 (tri methyl K4) peptide images

  • ab1342 (at 0.01 and 0.1 μg) used in Dot Blot to test for cross-reactivity of ab8580 (1/2500). An HRP-conjugated Mouse anti-rabbit polyclonal (1/10000) was used as the secondary antibody.

    See Abreview

References for Human Histone H3 (tri methyl K4) peptide (ab1342)

This product has been referenced in:

See all 10 Publications for this product

Product Wall

The sequence and purification method is propriety, but if you let me know what is important for you I can check if one of our product fits.

>The molecular weights of the different methylation states be extrememly difficult to distinguish as that type of modification creates a 14Dalton mass change.Currently, we do not offer full length methylated H3K4 proteins. We do have anEpiSeeker Histon...

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Peptides are available for both of these products. The peptide for ab8895,Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade, is product number ab1340 (http://www.abcam.com/histone-h3-peptide-mono-methyl-k4-ab1340.html).The peptide for ab8580,Anti-...

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Application Other

Review text: I used this product to do a dot blot specificity test for cross reactivity of modified histone antibodies and everything worked great. I made two dilutions of the peptide and spotted it on a nitrocellulose membrane using a Bio-Dot apparatus. Once the peptides were bound to the membrane I incubated with the appropriate primary and secondary antibodies at an assay dependent concentration and developed using ECL.
Sample: Human Purified protein

Primary antibody (in addition to 'Histone H3 peptide - tri methyl K4')
Primary antibody: Abcam primary antibody: Anti-Histone H3 (methylated) (tri methyl K4) antibody (ab8580)
Dilution: 1/2500

Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/10000
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Abcam user community

Verified customer

Submitted Nov 01 2010

We do not have a recommended protocol for Dot-blotting but can recommend trying a nitrocellulose membrane, a general manual will be able to help you. Specificity of those antibodies was tested by WB of Histone (ab121) (0.5µg per lane) and with block...

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The mechanism for coupling the peptides to beads via their terminal cys residue is based on a maleimide reaction scheme. The maleimide group of crosslinkers such as sulfo SMCC (PIERCE) or iodoacetyl moieties on an immobilisation matrix such as Sulfol...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"